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ktrD [2020-05-07 10:42:03]
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ktrD [2020-05-07 10:42:03]

glutamate-controlled potassium channel [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD], integral membrane subunit
locus
BSU_13500
pI
9.22
mw
49.26 kDa
protein length
449 aa Sequence Blast
gene length
1350 bp Sequence Blast
function
potassium uptake
product
glutamate-controlled potassium channel [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD], integral membrane subunit
essential
no
synonyms
ykrM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,416,067 1,417,416

    The protein

    Catalyzed reaction/ biological activity

  • uptake of potassium

    • Protein family

  • TrkH potassium transport family (with [protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], according to UniProt)

    • Paralogous protein(s)

  • [protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB]

    • Kinetic information

  • the [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|search|KtrD ]channel has a low affinity for potassium,this is determined by KtrD [pubmed|30753894]
  • the affinity of [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD] for potassium is strongly increased in the presence of glutamate (from 0.278 mM to 0.17 mM) [pubmed|32253343]

    • Effectors of protein activity

  • the affinity of [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD] for potassium is strongly increased in the presence of glutamate [pubmed|32253343]

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex, 36% identity) [Pubmed|23598340]

    • [SW|Localization]

  • integral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]
    description
    [Pubmed|12562800]

    regulation

  • constitutively expressed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B317 (ykrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1316 NBRP B. subtilis, Japan]
  • GHB12 (''ktrD''::''tet''), [Pubmed|12562800], available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s labs
  • GP2030 (''ktrD''::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • 1A956 (''ktrD''::''tet''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A956&Search=1A956 BGSC]
  • BKE13500 ([gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT
  • BKK13500 ([gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT

    • Expression vector

  • pGP2996: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP3465 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]
  • [SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]
  • [SW|João H Morais-Cabral], University of Porto, Portugal [https://www.i3s.up.pt/research-group?x=47 Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]

    • References

    Reviews

  • 21680052,27935846,25838295

    • Original publications

  • 12562800,23598340,28420751,28504641,30753894,32253343